5 research outputs found
ОПРЕДЕЛЯНЕ А ЕКСПРЕСИЯТА НА ФОСФАТИДИЛСЕРИН ПРИ СПЕРМАТОЗОИДИ В РАННА АПОПТОЗА ЧРЕЗ ФЛУОРЕСЦЕНТНО БИЛЯЗАН АНЕКСИН V
Double staining kit of Annexin V Cy3.18/6-CFDA was used to investigate the changes in phospholipide asymmetry after treating sperm cells with dexamethasone. The % of spermatozoa with registered translocation of PS in treated with dexamethazone groups at the 10-th min and in control no treated varied from 2.74%±0.65 to 2.30%±0.89. After the 5 hour of incubation these % increased to 39.83±3.33 for the treated group and 23.44±1.12 for the control. It was concluded that Annexin V binding assay is more sensitive in the detection of deterioration in membrane function than other conventional methods such as motility analysis and supravital techniques.Приложен е кит за двойно оцветяване с Анексин V Cy3.18 и 6-CFDA, даващ възможност за диференциране на апоптични от мъртви клетки. Процентът сперматозоиди с регистрирана експресия на ФС при свежи нетретирани сперматозоиди – контролна проба и експериментални, третирани с дексаметазон на 10-тата минута, варира от 2.30%±0.89 до 2.74%±0.65. След съхранение, на 5-я час този процент нараства до 23.44±1.12 за контролата и до 39.83±3.33 за опитната група. С настоящето изследване се доказва, че Анексин V теста е по-чувствителен за определяне промените в ПМ, в сравнение с конвенционалните методи на изследване за подвижност и преживяемост на
сперматозоидите
CELL DEATH DIFFERENTIATION IN BLACK HEADED RAMS SPERMATOZOA, USING FLUORESCENT LABELED ANNEXIN V
Double staining kit of Annexin V Cy3.18/6-CFDA was used to investigate the changes in phospholipide asymmetry after treating sperm cells with dexamethasone. The % of spermatozoa with registered translocation of PS in treated with dexamethazone groups at the 10-th min and in control no treated varied from 2.74%±0.65 to 2.30%±0.89. After the 5 hour of incubation these % increased to 39.83±3.33 for the treated group and 23.44±1.12 for the control. It was concluded that Annexin V binding assay is more sensitive in the detection of deterioration in membrane function than other conventional methods such as motility analysis and supravital techniques
ОПРЕДЕЛЯНЕ А ЕКСПРЕСИЯТА НА ФОСФАТИДИЛСЕРИН ПРИ СПЕРМАТОЗОИДИ В РАННА АПОПТОЗА ЧРЕЗ ФЛУОРЕСЦЕНТНО БИЛЯЗАН АНЕКСИН V
Double staining kit of Annexin V Cy3.18/6-CFDA was used to investigate the changes in phospholipide asymmetry after treating sperm cells with dexamethasone. The % of spermatozoa with registered translocation of PS in treated with dexamethazone groups at the 10-th min and in control no treated varied from 2.74%±0.65 to 2.30%±0.89. After the 5 hour of incubation these % increased to 39.83±3.33 for the treated group and 23.44±1.12 for the control. It was concluded that Annexin V binding assay is more sensitive in the detection of deterioration in membrane function than other conventional methods such as motility analysis and supravital techniques.Приложен е кит за двойно оцветяване с Анексин V Cy3.18 и 6-CFDA, даващ възможност за диференциране на апоптични от мъртви клетки. Процентът сперматозоиди с регистрирана експресия на ФС при свежи нетретирани сперматозоиди – контролна проба и експериментални, третирани с дексаметазон на 10-тата минута, варира от 2.30%±0.89 до 2.74%±0.65. След съхранение, на 5-я час този процент нараства до 23.44±1.12 за контролата и до 39.83±3.33 за опитната група. С настоящето изследване се доказва, че Анексин V теста е по-чувствителен за определяне промените в ПМ, в сравнение с конвенционалните методи на изследване за подвижност и преживяемост на
сперматозоидите
Generic model of an atom laser
We present a generic model of an atom laser by including a pump and loss term
in the Gross-Pitaevskii equation. We show that there exists a threshold for the
pump above which the mean matter field assumes a non-vanishing value in
steady-state. We study the transient regime of this atom laser and find
oscillations around the stationary solution even in the presence of a loss
term. These oscillations are damped away when we introduce a position dependent
loss term. For this case we present a modified Thomas-Fermi solution that takes
into account the pump and loss. Our generic model of an atom laser is analogous
to the semi-classical theory of the laser.Comment: 15 pages, including 5 figures, submitted to Phys. Rev. A, revised
manuscript, file also available at
http://www.physik.uni-ulm.de/quan/users/kne